Open AccessSuccessful delivery following intracytoplasmic sperm injection with calcium ionophore A23187 oocyte activation in a partially globozoospermic patient
Author(s) -
Kamiyama Hiroshi,
Shimizu Taketoshi,
Oki Tomomi,
Asada Terumi,
Araki Yasuyuki,
Araki Yasuhisa
Publication year - 2012
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1007/s12522-012-0123-z
Subject(s) - oocyte activation , intracytoplasmic sperm injection , oocyte , andrology , sperm , human fertilization , embryo , chemistry , biology , medicine , anatomy , microbiology and biotechnology , in vitro fertilisation
Purpose To describe a successful pregnancy outcome following intracytoplasmic sperm injection (ICSI) with assisted oocyte activation (AOA) in a case of partial globozoospermia. Methods AOA was accomplished with calcium ionophore A23187. Sperm morphology was observed via light, fluorescent and electron microscopy following a Diff‐Quik stain and fluorescein isothiocyanate‐labeled peanut agglutinin (FITC‐PNA) staining. An activation ability test was employed using a mouse oocyte exposed to strontium chloride. Results Via light microscopy, it was found that a large number of sperm possessed deficient acrosomes and a sharply rounded head; however, we observed both normal and the aforementioned abnormal sperm via FITC‐PNA staining of a semen specimen. Mouse oocyte activation was 87.5 % via natural activation without AOA. With AOA after ICSI, 100 % oocyte activation was observed. Five oocytes were retrieved, and AOA with A23187 after ICSI resulted in a high fertilization rate (4 of 5, 80 %). Two embryos developed and the patient subsequently delivered a healthy female infant without any congenital abnormalities. Conclusions We report a successful pregnancy outcome using an early stage embryo, which developed following ICSI using sperm from a partially globozoospermic patient who possessed temporary potential oocyte activation.