Open AccessEffect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD‐SCID mice
Author(s) -
AbdelGhani Mohammed Ali,
Abe Yasuyuki,
Asano Tomoyoshi,
Suzuki Hiroshi
Publication year - 2011
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1007/s12522-011-0091-8
Subject(s) - ovarian cortex , ovary , follicular phase , medicine , ovarian follicle , follicle , andrology , endocrinology , folliculogenesis , nod , transplantation , biology , embryo , cryopreservation , ovarian tissue , microbiology and biotechnology , diabetes mellitus
Purpose To investigate the effect of graft site and gonadotrophins administration on the number and survival rate of follicles of canine ovarian grafts transplanted to NOD‐severe combined immune deficiency (SCID) mice. Methods Fresh ovarian cortex slices obtained from immature bitches were grafted subcutaneously (SC), under kidney capsule (KC) or into ovarian bursa (OB) in NOD‐SCID mice. Two months after surgery, the mice allocated into non‐treated and treated gonadotrophins groups that injected with porcine follicle stimulating hormone during 7 days and human chorionic gonadotrophin 48 h later. Ovarian grafts were collected after 10 h of last injection and processed for histology. Results The number of transitional and preantral follicles under KC and into OB was significantly higher in gonadotrophins‐treated mice than those who received saline. Furthermore, the survival rates of primary, transitional and preantral follicles under KC and into OB grafts were significantly higher than those placed SC in the treated gonadotrophins group, and in the non‐treated gonadotrophins group; the proportion of primary and preantral follicle survival was significantly higher under KC and into OB than SC grafts. Conclusions In canine ovarian xenografting, administration of gonadotrophin could be effective for improvement of survival of transplanted ovary. Furthermore, the grafting into OB appeared to be better than grafting under KC, which in turn is better than SC.