Open AccessEmbryo development after intracytoplasmic sperm injection can be predicted by assessment of sperm nuclear chromatin
Author(s) -
Takayama Tomoko,
Katayose Haruo,
Yanagida Kaoru,
Sato Akira
Publication year - 2009
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1007/s12522-009-0010-4
Subject(s) - intracytoplasmic sperm injection , blastocyst , andrology , dna fragmentation , sperm , embryo , chromatin , acridine orange , embryogenesis , chemistry , biology , in vitro fertilisation , medicine , genetics , dna , staining , apoptosis , programmed cell death
Purpose To assess the influence of structural differences in sperm nuclei on embryo development in intracytoplasmic sperm injection (ICSI). Methods Semen obtained from forty‐four infertile patients who underwent ICSI was examined. In assessing blastocyst development, only those patients who had successfully obtained over five fertilized eggs were included to exclude any oocyte factors ( n = 22). Spermatozoa were assessed using excitation fluorescence flow cytometry with acridine orange and the sperm chromatin dispersion (SCD) test. Results There was a significant positive correlation between the COMP values obtained from flow cytometry and blastocyst formation. ( r = 0.477, p = 0.025). There was a significant negative correlation between the SCD values representing DNA fragmentation and blastocyst formation. ( r = 0.796, p < 0.001). COMP values and SCD values were independent parameters to assess sperm nuclear quality regarding embryo development in vitro ( r = 0.224, p = 0.080). Conclusion Results suggest that injection of spermatozoa with fewer disulfide bonds and less nuclear DNA fragmentation could achieve better blastocyst formation in human ICSI. Assessment of sperm chromatin should help to predict embryo development after ICSI.