Open AccessPre treatment with Bacillus subtilis mitigates drought induced photo-oxidative damages in okra by modulating antioxidant system and photochemical activity
Author(s) -
Pravisya Puthiyottil,
Yusuf Akkara
Publication year - 2021
Publication title -
physiology and molecular biology of plants/physiology and molecular biology of plants
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.754
H-Index - 35
eISSN - 0971-5894
pISSN - 0974-0430
DOI - 10.1007/s12298-021-00982-8
Subject(s) - glutathione reductase , superoxide dismutase , osmolyte , lipid peroxidation , chemistry , antioxidant , biochemistry , catalase , reactive oxygen species , glutathione , oxidative stress , chlorophyll , proline , enzyme assay , chlorophyll fluorescence , food science , glutathione peroxidase , enzyme , photosynthesis , organic chemistry , amino acid
Growth promoting potential of Bacillus subtilis (BS) in drought stressed Abelmoschus esculentus (L.) Moench (okra) was assessed by measuring the chlorophyll stability index (CSI), chlorophyll a (Chl-a) fluorescence, leaf osmotic potential and lipid peroxidation by malondialdehyde content, emission of reactive oxygen species (ROS), osmolyte content and the activity of non-enzyme and enzyme antioxidants. BS treatment significantly increased the leaf osmotic potential, osmolyte production and the activity of non-enzyme and enzyme antioxidants under drought stress. BS treatment mitigated the drought-induced reduction in Chl a fluorescence and CSI. Concomitant increase in total sugar, proline, non-enzyme antioxidants [glutathione and ascorbate] and enzyme antioxidants like superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase and dehydroascorbate reductase modulate the intracellular ROS concentration in okra to resist the stress induced oxidative damage in BS treated plants led to fast recovery and less photodamage.