Open AccessEffect of thermospermine on expression profiling of different gene using massive analysis of cDNA ends (MACE) and vascular maintenance in Arabidopsis
Author(s) -
G. H. M. Sagor,
Stefan Simm,
Dong Wook Kim,
Masaru Niitsu,
Tomonobu Kusano,
Thomas Berberich
Publication year - 2021
Publication title -
physiology and molecular biology of plants/physiology and molecular biology of plants
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.754
H-Index - 35
eISSN - 0971-5894
pISSN - 0974-0430
DOI - 10.1007/s12298-021-00967-7
Subject(s) - arabidopsis , mutant , biology , catabolism , gene , complementary dna , microbiology and biotechnology , gene expression , vascular tissue , polyamine oxidase , gene expression profiling , arabidopsis thaliana , biochemistry , botany , metabolism , enzyme , putrescine
Arabidopsis thaliana polyamine oxidase 5 gene ( AtPAO5 ) functions as a thermospermine (T-Spm) oxidase. Aerial growth of its knock-out mutant ( Atpao5-2 ) was significantly repressed by low dose(s) of T-Spm but not by other polyamines. To figure out the underlying mechanism, massive analysis of 3'-cDNA ends was performed. Low dose of T-Spm treatment modulates more than two fold expression 1,398 genes in WT compared to 3186 genes in Atpao5-2. Cell wall, lipid and secondary metabolisms were dramatically affected in low dose T-Spm-treated Atpao5-2, in comparison to other pathways such as TCA cycle-, amino acid- metabolisms and photosynthesis. The cell wall pectin metabolism, cell wall proteins and degradation process were highly modulated. Intriguingly Fe-deficiency responsive genes and drought stress-induced genes were also up-regulated, suggesting the importance of thermospermi'ne flux on regulation of gene network. Histological observation showed that the vascular system of the joint part between stem and leaves was structurally dissociated, indicating its involvement in vascular maintenance. Endogenous increase in T-Spm and reduction in H 2 O 2 contents were found in mutant grown in T-Spm containing media. The results indicate that T-Spm homeostasis by a fine tuned balance of its synthesis and catabolism is important for maintaining gene regulation network and the vascular system in plants.