Open AccessModulation of growth and key physiobiochemical attributes after foliar application of zinc sulphate (ZnSO4) on wheat (Triticum aestivum L.) under cadmium (Cd) stress
Author(s) -
Shagufta Perveen,
Muhammad Saeed,
Abida Parveen,
Muhammad Tariq Javed,
Sara Zafar,
Naeem Iqbal
Publication year - 2020
Publication title -
physiology and molecular biology of plants/physiology and molecular biology of plants
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.754
H-Index - 35
eISSN - 0971-5894
pISSN - 0974-0430
DOI - 10.1007/s12298-020-00861-8
Subject(s) - cadmium , zinc , chemistry , agronomy , stress (linguistics) , horticulture , botany , biology , organic chemistry , philosophy , linguistics
A pot experiment was conducted to examine the effect of foliar application of various levels of ZnSO 4 on wheat ( Triticum aestivum L.) under cadmium (Cd) stress. Seeds of two wheat varieties i.e., Ujala-2016 and Anaj-2017 were sown in sand filled plastic pots. Cadmium (CdCl 2 ) stress i.e., 0 and 0.5 mM CdCl 2 was applied in full strength Hoagland's nutrient solution after 4 weeks of seed germination. Foliar spray of varying ZnSO 4 levels i.e., 0, 2, 4, 6 and 8 mM was applied after 2 weeks of CdCl 2 stress induction (of 6 week old plants). After 3 weeks of foliar treatment leaf samples of 9 week old wheat plants were collected for the determination of changes in various growth and physiobiochemical attributes. Results obtained showed that cadmium stress (0.5 mM CdCl 2 ) significantly decreased shoot and root fresh and dry weights, shoot and root lengths, yield attributes, chlorophyll a contents and total phenolics, while increased hydrogen peroxide (H 2 O 2 ), total soluble proteins, free proline, glycinebetaine (GB) contents, and activities of antioxidant enzymes i.e., catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD). Foliar application of varying ZnSO 4 levels significantly increased various growth attributes, chlorophyll b contents, H 2 O 2 , free proline, GB and activities of antioxidant enzymes i.e., CAT, POD and APX, while decreased relative water contents and total phenolics under Cd stress or non stress conditions. Furthermore, both wheat varieties showed differential response under Cd stress and towards foliar application of ZnSO 4 e.g., wheat var. Ujala-2016 was higher in shoot dry weight, root length, root fresh and dry weights, total leaf area per plant, 100 grains weight, number of tillers per plant, chlorophyll b , hydrogen peroxide (H 2 O 2 ), activities of APX, POD, glycinebetaine and leaf free proline contents, while var. Anaj-2017 exhibited high shoot fresh weight, grain yield per plant, no. of grains per plant, chlorophyll contents, chlorophyll a/b ratio, total phenolics, MDA and total soluble protein contents under cadmium stress or non stress conditions.