Open AccessAluminium accumulation in excess and related anti-oxidation responses in C4 weed (Amaranthus viridis L.)
Author(s) -
Bipul Sarkar,
Indraneel Saha,
Arnab Kumar De,
Arijit Ghosh,
Malay Kumar Adak
Publication year - 2020
Publication title -
physiology and molecular biology of plants/physiology and molecular biology of plants
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.754
H-Index - 35
eISSN - 0971-5894
pISSN - 0974-0430
DOI - 10.1007/s12298-020-00840-z
Subject(s) - bioaccumulation , chemistry , proline , abts , catalase , oxidative stress , hydrogen peroxide , peroxidase , food science , ferric , antioxidant , botany , biochemistry , dpph , biology , enzyme , amino acid , organic chemistry
C 4 species, Amaranthus viridis L. exhibited a significant bioaccumulation of aluminium (Al) through the duration of 3- and 5-days exposure. As compared to control, Amaranthus appeared as excess-accumulator with maximum 5.85-fold bioaccumulation of Al in root. Cellular responses to Al tolerance initially scored tissue specific distribution of metal through cortical layers revealed by electron microscopy. The affected cells changed an oxidative status as read by histochemical stains, particularly, for hydrogen peroxide. Osmotic stress and its stability were scored by maximum proline and free amino acids accumulation with 1.53 and 1.59-fold increase over control. The accumulation of phenolics and flavonoids were over expressed in the ranges of 2.48-2.50-fold and 2.00-1.5-fold at 3- and 5-days respectively against control. Anti-oxidation to detoxify Al stress was facilitated by variants of peroxidases. For exclusion mechanism of metal, esterase activity significantly over expressed with maximum value of 1.80-fold at 5-days. The polymorphism of esterase exhibited few significant over produced bands, varied in numbers as detected by densitometric scanning. Moreover, plant extract was satisfactorily potential under in vitro anti-oxidation systems through assay of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), ferric chelation activity etc. Therefore, weeds like Amaranthus would be a bioprospecting in role likely involved in phytoremediation of metal.