Open AccessAssessment of FTO Gene Polymorphism and its Association with Type 2 Diabetes Mellitus in North Indian Populations
Author(s) -
Kahkashan Naaz,
Amit Kumar,
Ipsita Choudhury
Publication year - 2018
Publication title -
indian journal of clinical biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 42
ISSN - 0970-1915
DOI - 10.1007/s12291-018-0778-2
Subject(s) - type 2 diabetes mellitus , polymorphism (computer science) , gene , diabetes mellitus , genetics , medicine , type 2 diabetes , biology , endocrinology , genotype
FTO gene polymorphism related to type 2 diabetes and obesity was studied in this north Indian population. This study was done, due to a continuous increase in the risk of obesity and type 2 diabetes in north Indian population, because of lifestyle and genetic variations. Clinically diagnosed subjects of type 2 diabetes mellitus (as per ADA criteria) were taken as cases and age and sex matched subjects without any associated illness were taken as controls. Obesity was estimated by calculating waist circumference and BMI in the study cases and controls. For genetic variation, DNA was isolated with Quaigen kit method and isolated DNA was amplified with PCR. Amplified DNA was resolved in 1% agarose gel electrophoresis. The Hardy-Weinberg equilibrium, OR, CI and P value were calculated using standard protocols. FTO gene polymorphism (SNP 9940128) was found to be significantly correlated with type 2 diabetes mellitus and obesity. The AG genotype frequency was observed to be higher (13.09%) with ( P < 0.0001) in the cases as compared to controls. Logistic regression analysis was conducted for AG and GG genotypes with respect to AA. In this novel study genetic co-relation was observed between FTO gene polymorphisms and type 2 diabetes and obesity in the north Indian population.