Open AccessDiabetic Nephropathy Assessment: Microtubule-Associated Protein 1 Light-Chain 3B a New Promising Biomarker
Author(s) -
Mervat S. Mohamed,
Sanaa Eissa,
Mona Mostafa,
Marwa G. A. Hegazy
Publication year - 2018
Publication title -
indian journal of clinical biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 42
ISSN - 0970-1915
DOI - 10.1007/s12291-018-0773-7
Subject(s) - albuminuria , biomarker , diabetic nephropathy , autophagy , medicine , urinary system , pathogenesis , urine , receiver operating characteristic , diabetes mellitus , urology , oncology , endocrinology , kidney , biology , apoptosis , biochemistry
Autophagy is a highly conserved pathway. Impairment of autophagy is implicated in the pathogenesis of diabetic nephropathy. The current study applied a bioinformatics analysis to retrieve promising autophagy biomarker relevant diabetic nephropathy. Urinary expression of Microtubule-associated protein 1 light-chain 3B (LC3B) RNA was assessed. Urine samples of 86 type II diabetic kidney disease Egyptian patients (albuminuria group) were provided to quantify urinary expression of LC3B. A group of 30 healthy volunteers were also enrolled in addition to non-albuminuria group including 44 patients. Our study revealed a cut-off value for urinary LC3B expression level that was calculated by receiver-operating characteristic curve as 0.866. Sensitivity and specificity of LC3B were 83.7 and 78.4% respectively. The positivity rate of urinary LC3B expression level was significantly lower in diabetic nephropathy patients than control group. LC3B has great clinical value as promising biomarker in diabetic nephropathy assessment.