Open AccessChanges in Complement Levels and Activity of Red Blood Cells, Fresh Frozen Plasma, and Platelet Concentrates During Storage
Author(s) -
Xiaomin Liu,
Ruoshuai Cui,
Yingwei Song,
Yang Liu,
Leiying Zhang,
Shufang Wang,
Hongfei Zhang,
Jinghua Sun,
Ping Di,
Yang Yu
Publication year - 2020
Publication title -
indian journal of hematology and blood transfusion/indian journal of hematology and blood transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.213
H-Index - 15
eISSN - 0974-0449
pISSN - 0971-4502
DOI - 10.1007/s12288-020-01338-0
Subject(s) - platelet , complement system , fresh frozen plasma , whole blood , complement (music) , blood preservation , transfusion medicine , platelet rich plasma , blood transfusion , medicine , immunology , chemistry , andrology , antibody , biochemistry , complementation , gene , phenotype
Complement cascade plays an important role in the field of transfusion medicine. The study aimed to detect the complement levels of different blood components and different blood types to explore the risk of transfusion of stored blood. The samples including red blood cells (n = 110), fresh frozen plasma (n = 120), and platelet concentrates (n = 104) from healthy blood donors in our center were collected. Complement components (C3, C4, C3b, C3d, and CH50) were assayed to evaluate the activation of complement. The complement levels of various blood components at different storage times were observed. The differences in complement levels of four blood types in various blood components were compared. The complement levels of red blood cells in storage were low, with no significant changes ( P > 0.05). C3b and C3d levels in platelets began to significantly increase after storage for 3 days ( P < 0.05). The fresh frozen plasma during storage had higher complement levels, and the concentrations of C3 and C4 decreased and C3b and C3d increased at month 4 ( P < 0.05). The differences in complement levels of four blood types in various blood components did not significantly change ( P > 0.05), but the C3b and C3d levels of AB fresh frozen plasma remained stable during storage, which different from other blood types. The transfusion of red blood cells was relatively safe in terms of complement activation. The activation of complement proteins occurred during the storage of platelet and plasma, except group AB plasma.