Open AccessGlucose Protects Cochlear Hair Cells Against Oxidative Stress and Attenuates Noise-Induced Hearing Loss in Mice
Author(s) -
Hao Xiong,
Lan Lai,
Yongyi Ye,
Yiqing Zheng
Publication year - 2021
Publication title -
neuroscience bulletin/neuroscience bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.54
H-Index - 47
eISSN - 1673-7067
pISSN - 1995-8218
DOI - 10.1007/s12264-020-00624-1
Subject(s) - oxidative stress , hair cell , reactive oxygen species , noise induced hearing loss , hearing loss , cochlea , oxidative phosphorylation , microbiology and biotechnology , glucose transporter , auditory fatigue , chemistry , biology , endocrinology , medicine , noise exposure , biochemistry , neuroscience , audiology , insulin
Oxidative stress is the key determinant in the pathogenesis of noise-induced hearing loss (NIHL). Given that cellular defense against oxidative stress is an energy-consuming process, the aim of the present study was to investigate whether increasing energy availability by glucose supplementation protects cochlear hair cells against oxidative stress and attenuates NIHL. Our results revealed that glucose supplementation reduced the noise-induced formation of reactive oxygen species (ROS) and consequently attenuated noise-induced loss of outer hair cells, inner hair cell synaptic ribbons, and NIHL in CBA/J mice. In cochlear explants, glucose supplementation increased the levels of ATP and NADPH, as well as attenuating H 2 O 2 -induced ROS production and cytotoxicity. Moreover, pharmacological inhibition of glucose transporter type 1 activity abolished the protective effects of glucose against oxidative stress in HEI-OC1 cells. These findings suggest that energy availability is crucial for oxidative stress resistance and glucose supplementation offers a simple and effective approach for the protection of cochlear hair cells against oxidative stress and NIHL.