Open AccessDivergent Projection Patterns Revealed by Reconstruction of Individual Neurons in Orbitofrontal Cortex
Author(s) -
Junjun Wang,
Pei Sun,
Xiaohua Li,
Sen Jin,
Anan Li,
Jianxia Kuang,
Ning Li,
Yadong Gang,
Rui Guo,
Shaoqun Zeng,
Fuqiang Xu,
Yuhui Zhang
Publication year - 2020
Publication title -
neuroscience bulletin/neuroscience bulletin
Language(s) - English
Resource type - Journals
eISSN - 1673-7067
pISSN - 1995-8218
DOI - 10.1007/s12264-020-00616-1
Subject(s) - orbitofrontal cortex , neuroscience , projection (relational algebra) , cortex (anatomy) , population , cerebral cortex , biology , computer science , prefrontal cortex , medicine , cognition , algorithm , environmental health
The orbitofrontal cortex (OFC) is involved in diverse brain functions via its extensive projections to multiple target regions. There is a growing understanding of the overall outputs of the OFC at the population level, but reports of the projection patterns of individual OFC neurons across different cortical layers remain rare. Here, by combining neuronal sparse and bright labeling with a whole-brain florescence imaging system (fMOST), we obtained an uninterrupted three-dimensional whole-brain dataset and achieved the full morphological reconstruction of 25 OFC pyramidal neurons. We compared the whole-brain projection targets of these individual OFC neurons in different cortical layers as well as in the same cortical layer. We found cortical layer-dependent projections characterized by divergent patterns for information delivery. Our study not only provides a structural basis for understanding the principles of laminar organizations in the OFC, but also provides clues for future functional and behavioral studies on OFC pyramidal neurons.