Open AccessTranscriptional regulation by HSV-1 induced HTRP via acetylation system
Author(s) -
Jie Chen,
Yanmei Li,
Jianfeng Li,
Long-ding Liu,
Yun Liao,
Ruixiong Na,
Jingjing Wang,
Lichun Wang,
Qihan Li
Publication year - 2010
Publication title -
virologica sinica
Language(s) - English
Resource type - Journals
eISSN - 1674-0769
pISSN - 1995-820X
DOI - 10.1007/s12250-010-3147-8
Subject(s) - acetylation , transcription (linguistics) , histone , luciferase , transcriptional regulation , chemistry , lysine , herpes simplex virus , gene , repressor , enzyme , biology , microbiology and biotechnology , biochemistry , transcription factor , virus , virology , transfection , amino acid , philosophy , linguistics
The protein HTRP (human transcription regulator protein) is encoded by the differential gene htrp and induced by Herpes simplex virus type 1 (HSV-1) infection in KMB-17 cells. HTRP was found to interact with SAP30 (mSin3A Association Protein), one of the components of co-repressor complex mSin3A, which is part of the deacetylation transfer enzyme HDAC. To reveal the biological significance of the interaction between HTRP and SAP30, real- time PCR and a dual-luciferase detecting system was used. The results indicate that HTRP could inhibit the transcription of a viral promoter, whose interaction with SAP30 synergistically affects transcriptional inhibition of the viral genes, and is related to HDAC enzyme activity. ChIP experiments demonstrate that HTRP could promote HDAC activity by increasing the deacetylation level of lysine 14 and lysine 9 in histone H3.
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