Open AccessOptimization of a Clinically Relevant Chemical-Mechanical Tissue Dissociation Workflow for Single-Cell Analysis
Author(s) -
E. Celeste Welch,
Harry Yu,
Anubhav Tripathi
Publication year - 2021
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-021-00667-y
Subject(s) - dissociation (chemistry) , chemistry , biomedical engineering , flow cytometry , biophysics , materials science , microbiology and biotechnology , medicine , biology
While single-cell analysis technology has flourished, obtaining single cells from complex tissues continues to be a challenge. Current methods require multiple steps and several hours of processing. This study investigates chemical and mechanical methods for clinically relevant preparation of single-cell suspension from frozen biopsy cores of complex tissues. The developed protocol can be completed in 15 min.