Open AccessGelatin Promotes Cell Retention Within Decellularized Heart Extracellular Matrix Vasculature and Parenchyma
Author(s) -
Karis R Tang-Quan,
Yutao Xi,
Camila HochmanMendez,
Qian Xiang,
Po-Feng Lee,
Luiz C. Sampaio,
Doris A. Taylor
Publication year - 2020
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-020-00634-z
Subject(s) - decellularization , gelatin , extracellular matrix , microbiology and biotechnology , parenchyma , cell , extracellular , biomedical engineering , chemistry , medicine , pathology , biology , biochemistry
Recellularization of organ decellularized extracellular matrix (dECM) offers a potential solution for organ shortage in allograft transplantation. Cell retention rates have ranged from 10 to 54% in varying approaches for reseeding cells in whole organ dECM scaffolds. We aimed to improve recellularization by using soluble gelatin as a cell carrier to deliver endothelial cells to the coronary vasculature and cardiomyocytes to the parenchyma in a whole decellularized rat heart.