Open AccessA Chemically Defined Common Medium for Culture of C2C12 Skeletal Muscle and Human Induced Pluripotent Stem Cell Derived Spinal Spheroids
Author(s) -
Rachel R. Besser,
Annie C. Bowles,
Ahmad Alassaf,
Daniel Carbonero,
Roseli Martins Tristão Maciel,
Mario Saporta,
Ashutosh Agarwal
Publication year - 2020
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-020-00624-1
Subject(s) - myogenesis , induced pluripotent stem cell , skeletal muscle , c2c12 , microbiology and biotechnology , myocyte , cell culture , stem cell , chemically defined medium , biology , human induced pluripotent stem cells , neuroscience , anatomy , in vitro , genetics , embryonic stem cell , gene
Multicellular platforms and linked multi organ on chip devices are powerful tools for drug discovery, and basic mechanistic studies. Often, a critical constraint is defining a culture medium optimal for all cells present in the system. In this study, we focused on the key cells of the neuromuscular junction i.e., skeletal muscle and motor neurons.