Open AccessCyclic Strain Promotes H19 Expression and Vascular Tube Formation in iPSC-Derived Endothelial Cells
Author(s) -
Mark J Vander Roest,
W. David Merryman
Publication year - 2020
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-020-00617-0
Subject(s) - brachyury , microbiology and biotechnology , induced pluripotent stem cell , cellular differentiation , cd31 , embryonic stem cell , klf4 , umbilical vein , gene expression , mesoderm , biology , chemistry , angiogenesis , gene , cancer research , genetics , in vitro
Induced pluripotent stem cell (iPSC)-derived endothelial cells (ECs) have the potential for therapeutic application in several cardiovascular diseases. Mechanical strain is known to regulate EC behavior and stem cell differentiation and may play a role in directing EC differentiation of iPSCs. H19 , a long non-coding RNA (lncRNA), is known to affect ECs in several mechanically relevant pathologies and may play a role in this process as well. Therefore, we investigated expression changes of H19 resulting from mechanical stimulation during EC differentiation, as well as functional effects on EC tube formation.