Open AccessGenome Engineering Renal Epithelial Cells for Enhanced Volume Transport Function
Author(s) -
Matthew H. Wilson,
Ruth Ann Veach,
Weijia Luo,
Richard C. Welch,
Shuvo Roy,
William H. Fissell
Publication year - 2019
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-019-00601-3
Subject(s) - flow cytometry , microbiology and biotechnology , biotinylation , biology , aquaporin 1 , hek 293 cells , cell culture , genetics , engineering , inlet , mechanical engineering , water channel
Bioengineering an implantable artificial kidney (IAK) will require renal epithelial cells capable of reabsorption of salt and water. We used genome engineering to modify cells for improved Na + /H + exchange and H 2 O reabsorption. The non-viral piggyBac transposon system enables genome engineering cells to stably overexpress one or more transgenes simultaneously.