Open AccessOptogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization
Author(s) -
Stephanie H. Geller,
Enoch Antwi,
Barbara Di Ventura,
Megan N. McClean
Publication year - 2019
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-019-00598-9
Subject(s) - repressor , biology , saccharomyces cerevisiae , psychological repression , gene expression , regulation of gene expression , gene , genetics , derepression , microbiology and biotechnology , computational biology
Controlling gene expression is a fundamental goal of basic and synthetic biology because it allows insight into cellular function and control of cellular activity. We explored the possibility of generating an optogenetic repressor of gene expression in the model organism Saccharomyces cerevisiae by using light to control the nuclear localization of nuclease-dead Cas9, dCas9.