Open AccessShear Stress in Bone Marrow has a Dose Dependent Effect on cFos Gene Expression in In Situ Culture
Author(s) -
Kimberly J. Curtis,
Thomas R. Coughlin,
M. Alyssa Varsanik,
Glen L. Niebur
Publication year - 2019
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-019-00594-z
Subject(s) - mechanotransduction , bone marrow , stimulation , osteocyte , microbiology and biotechnology , shear stress , chemistry , gene expression , bone remodeling , biophysics , osteoblast , anatomy , pathology , biology , in vitro , endocrinology , materials science , medicine , gene , biochemistry , composite material
Mechanical stimulation of bone is necessary to maintain its mass and architecture. Osteocytes within the mineralized matrix are sensors of mechanical deformation of the hard tissue, and communicate with cells in the marrow to regulate bone remodeling. However, marrow cells are also subjected to mechanical stress during whole bone loading, and may contribute to mechanically regulated bone physiology. Previous results from our laboratory suggest that mechanotransduction in marrow cells is sufficient to cause bone formation in the absence of osteocyte signaling. In this study, we investigated whether bone formation and altered marrow cell gene expression response to stimulation was dependent on the shear stress imparted on the marrow by our loading regime.