Open AccessInjectable, Hyaluronic Acid-Based Scaffolds with Macroporous Architecture for Gene Delivery
Author(s) -
Arshia Ehsanipour,
Tommy Nguyen,
Tasha Aboufadel,
Mayilone Sathialingam,
Phillip Cox,
Weikun Xiao,
Christopher M. Walthers,
Stephanie K. Seidlits
Publication year - 2019
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-019-00593-0
Subject(s) - gene delivery , self healing hydrogels , hyaluronic acid , transgene , chemistry , in vivo , tissue engineering , reporter gene , peg ratio , genetic enhancement , biomedical engineering , microbiology and biotechnology , gene expression , materials science , biophysics , biochemistry , biology , gene , anatomy , finance , medicine , organic chemistry , economics
Biomaterials can provide localized reservoirs for controlled release of therapeutic biomolecules and drugs for applications in tissue engineering and regenerative medicine. As carriers of gene-based therapies, biomaterial scaffolds can improve efficiency and delivery-site localization of transgene expression. Controlled delivery of gene therapy vectors from scaffolds requires cell-scale macropores to facilitate rapid host cell infiltration. Recently, advanced methods have been developed to form injectable scaffolds containing cell-scale macropores. However, relative efficacy of in vivo gene delivery from scaffolds formulated using these general approaches has not been previously investigated. Using two of these methods, we fabricated scaffolds based on hyaluronic acid (HA) and compared how their unique, macroporous architectures affected their respective abilities to deliver transgenes via lentiviral vectors in vivo .