Open AccessImmunomodulatory Effects of Human Cryopreserved Viable Amniotic Membrane in a Pro-Inflammatory Environment In Vitro
Author(s) -
Claire E. Witherel,
Tony Yu,
Mark Concan,
Will Dampier,
Kara L. Spiller
Publication year - 2017
Publication title -
cellular and molecular bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.668
H-Index - 34
eISSN - 1865-5033
pISSN - 1865-5025
DOI - 10.1007/s12195-017-0494-7
Subject(s) - downregulation and upregulation , macrophage , inflammation , angiogenesis , m2 macrophage , tumor necrosis factor alpha , immunology , microbiology and biotechnology , biology , in vitro , chemistry , cancer research , biochemistry , gene
Chronic wounds remain a major clinical challenge. Human cryopreserved viable amniotic membrane (hCVAM) is among the most successful therapies, but the mechanisms of action remain loosely defined. Because proper regulation of macrophage behavior is critical for wound healing with biomaterial therapies, we hypothesized that hCVAM would positively regulate macrophage behavior in vitro , and that soluble factors released from the hCVAM would be important for this effect.