Open AccessER stress-related molecules induced by Hantaan virus infection in differentiated THP-1 cells
Author(s) -
Zhuo Li,
YuTing Shen,
Yun Song,
Yusi Zhang,
Chunmei Zhang,
Ying Ma,
Fanglin Zhang,
Lihua Chen
Publication year - 2021
Publication title -
cell stress and chaperones
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.994
H-Index - 87
eISSN - 1466-1268
pISSN - 1355-8145
DOI - 10.1007/s12192-020-01150-9
Subject(s) - unfolded protein response , hantaan virus , endoplasmic reticulum , biology , apoptosis , protein kinase r , atf6 , messenger rna , virology , glucose regulated protein , virus , microbiology and biotechnology , kinase , protein kinase a , gene , biochemistry , mitogen activated protein kinase kinase , hantavirus
Endoplasmic reticulum stress (ER stress) can be induced by virus infection. In this part, we explored whether Hantaan virus (HTNV) infection could induce ER stress in differentiated THP-1 (dTHP-1) cells. It showed that the mRNA and protein levels of ER stress-related 78 kDa glucose-regulated protein (GRP78, HSPA5) and mRNA levels of X box-binding protein 1 (XBP-1), activating transcription factor 6(ATF6) and PKR-like ER kinase (PERK) after HTNV infection, were significantly higher than that in uninfected control group. However, the mRNA levels of C/EBP homologous protein (CHOP), glucose-regulated protein 94 (GRP94, HSPC4), and inositol-requiring enzyme1 (IRE1) were not significantly different between the infected group and the untreated group in 2 h after virus infection. It is unusual in activating GRP78 but not GRP94. Meanwhile, dTHP-1 cells infected with HTNV at 12 h did not show obvious apoptosis. These results indicated that the HTNV infection could induce the unfolded protein response (UPR) in dTHP-1 cells, without directly leading to cell apoptosis during 12 h after virus infection.