Open AccessAntimicrobial Activity and Mechanism of Essential Oil of Endemic Salvia hypargeia Finc. & Mey. in Turkey
Author(s) -
Elif Ayşe Erdoğan Eliuz
Publication year - 2021
Publication title -
indian journal of microbiology/indian journal of microbiology (print)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 46
eISSN - 0973-7715
pISSN - 0046-8991
DOI - 10.1007/s12088-021-00939-1
Subject(s) - antimicrobial , essential oil , camphor , acinetobacter baumannii , chemistry , traditional medicine , microbiology and biotechnology , broth microdilution , agar , microorganism , food science , biology , bacteria , minimum inhibitory concentration , pseudomonas aeruginosa , medicine , genetics
In this study, the aerial parts of Salvia hypargeia were subjected to hydrodistillation and the resulting compounds were analyzed in GC-MS. Antimicrobial activity of S. hypargeia essential oil (EO) against A. baumannii , S. aureus , and C. tropicalis were determined by agar well diffusion assay and microdilution method. Antimicrobial mechanism of the EO were investigated based on change of TTC-dehydrogenase relative, leakages of intracellular protein, DNA and potassium ion (K +). The main components of the EO were β-pinene, 1,8-cineole, camphor, α-pinene, 4-terpineol, and 4-thujanol. The MICs of the EO against the microorganisms were 15.2 mg/mL for S. aureus, 17.5 mg/mL for C. tropicalis and 28.8 mg/mL for A. baumannii. The inhibition zones were 18.16 mm, 25.01 mm, and 27.01 mm for A. baumannii , S. aureus , and C. tropicalis, respectively ( p < 0.05). An increase in DNA, protein and K + leakage was observed when microorganisms were exposed to the EO. The TTC-DRA of the treated microorganism cells was also significantly decreased because of slowing the respiration. The present study provided an experimental basis of practical application of S. hypargeia EO as a natural agent.