Open AccessOptimization of Cr6+ Removal by Bacillus subtilis Strain SZMC 6179J from Chromium-Containing Soil
Author(s) -
Jianghong Liu,
Jian Xue,
Xiaohang Wei,
Huimin Su,
Ruidan Xu
Publication year - 2020
Publication title -
indian journal of microbiology/indian journal of microbiology (print)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 46
eISSN - 0973-7715
pISSN - 0046-8991
DOI - 10.1007/s12088-020-00886-3
Subject(s) - bacillus subtilis , strain (injury) , response surface methodology , chromium , adsorption , chemistry , 16s ribosomal rna , inoculation , microbiology and biotechnology , nuclear chemistry , bacteria , chromatography , biology , biochemistry , horticulture , genetics , organic chemistry , anatomy , gene
The removal rate of Cr 6+ has been explored by the optimized removal conditions. Five Cr-resistant strains were isolated from chromium-contained soil. The most efficient strain S1 was identified as Bacillus subtilis strain SZMC 6179J through 16S rDNA. Response surface methodology (RSM) was used to investigate the effects of four independent variables, including initial pH, initial Cr 6+ concentration (mg/L), time (h) and inoculation percentage (%). RSM revealed that when pH was 5.02, time was 24.0 h, inoculation percentage was 4.64% (v/v) and initial concentration of Cr 6+ was 55.0 mg/L, the optimal condition was obtained. Under the optimum conditions, the actual response values for Bacillus subtilis strain SZMC 6179J was 93.50%. The pH was the most significant factor towards removal rate of Cr 6+ . The result showed that the removal mechanism of Cr 6+ by Bacillus subtilis strain SZMC 6179J was reduction under normal conditions. The removal mechanism of Cr 6+ by Bacillus subtilis strain SZMC 6179J was adsorption under adverse conditions.