Open AccessEfficacy of a Ruminal Bacteriocin Against Pure and Mixed Cultures of Bovine Mastitis Pathogens
Author(s) -
Fernanda Godoy-Santos,
Maximiliano Soares Pinto,
Ana Andréa Teixeira Barbosa,
Maria Aparecida Vasconcelos Paiva Brito,
Hilário Cuquetto Mantovani
Publication year - 2019
Publication title -
indian journal of microbiology/indian journal of microbiology (print)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 46
eISSN - 0973-7715
pISSN - 0046-8991
DOI - 10.1007/s12088-019-00799-w
Subject(s) - mastitis , microbiology and biotechnology , bacteriocin , biology , streptococcus agalactiae , antibiotics , udder , veterinary medicine , streptococcus , bacteria , medicine , antimicrobial , genetics
Bacteriocins have been suggested as an alternative to conventional antibiotics for the prevention and treatment of mastitis infections. Predominant bacteria associated with bovine mastitis (n = 276 isolates) were evaluated for their susceptibility to bovicin HC5, a ruminal bacteriocin produced by Streptococcus equinus HC5. Bovicin HC5 inhibited most (> 80%) of the streptococcal and staphylococcal strains tested, but showed no effect against Escherichia coli strains. Susceptibility and resistance testing indicated that approximately 95% of the S. aureus strains were inhibited by concentrations of bovicin HC5 varying from 40 to 2560 AU ml -1 . Bovicin HC5 (62.50 AU ml -1 ) also inhibited the growth of aerobic and anaerobic mixed cultures of S. aureus and S. agalactiae , but the combination with 0.25 mmol l -1 of EDTA showed even greater bactericidal activity. These results demonstrate that bovicin HC5 is effective against the most prevalent pathogens found in contagious udder infections and could complement the use antibiotics in mastitis prophylaxis and therapy.