Open AccessInfluence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids
Author(s) -
Sanjay Patel,
Rahul Gupta,
Virendra Kumar,
Primata Mardina,
Rowina Lestari,
Vipin Chandra Kalia,
Myung–Seok Choi,
Jung-Kul Lee
Publication year - 2019
Publication title -
indian journal of microbiology/indian journal of microbiology (print)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 46
eISSN - 0973-7715
pISSN - 0046-8991
DOI - 10.1007/s12088-019-00796-z
Subject(s) - metal ions in aqueous solution , chemistry , zinc , metal , inorganic ions , thermal stability , enzyme , immobilized enzyme , hybrid , copper , hybrid material , ion , biochemistry , organic chemistry , biology , botany
Immobilization of enzymes through metal-based system is demonstrated as a promising approach to enhance its properties. In this study, the influence of metals ions, including copper, cobalt and zinc (Zn) on the immobilization of β-glucosidase (BGL) through the synthesis of protein-inorganic hybrid was evaluated at 4 °C. Among these metal ions-based hybrids, Zn showed the highest encapsulation yield and relative activity of 87.5 and 207%, respectively. Immobilized BGL exhibited higher pH and temperature stability compared to free form. Thermal stability of hybrid improved up to 26-fold at 60 °C. After 10 cycles of reuse, immobilized enzyme retained 93.8% of residual activity. These results suggested that metal ions played a significant role in the enzyme immobilization as a protein-inorganic hybrid. Overall, this strategy can be potentially applied to enhance the properties of enzymes though effective encapsulation for the broad biotechnological applications.