Open AccessCartilage Regeneration of Adipose-Derived Stem Cells in the TGF-β1-Immobilized PLGA-Gelatin Scaffold
Author(s) -
Feng Yin,
Jianping Cai,
Wen Zen,
Yanhui Wei,
Wei Zhou,
Feng Yuan,
Shree Ram Singh,
Yiyong Wei
Publication year - 2014
Publication title -
stem cell reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.247
H-Index - 73
eISSN - 1558-6804
pISSN - 1550-8943
DOI - 10.1007/s12015-014-9561-9
Subject(s) - chondrogenesis , scaffold , cartilage , regeneration (biology) , gelatin , chemistry , tissue engineering , biomedical engineering , microbiology and biotechnology , plga , adipose tissue , stem cell , glycosaminoglycan , anatomy , medicine , biology , biochemistry , in vitro
Articular cartilage has restricted self-regenerative capacity; therefore, treatment of cartilage lesions is a great challenge in the field of orthopedics. In the present study, we evaluate the enhancing effect of a transforming growth factor-beta 1 (TGF-β1)-immobilized scaffold, fabricated by incorporating TGF-β1-loaded gelatin microspheres into PLGA framework, on the differentiation of adipose-derived stem cells (ASCs) into chondrocytes. Significant increase in cell proliferation was observed in the TGF-β1-immobilized PLGA-gelatin scaffold, as compared with the ASC-seeded non-TGF-β1-immobilized PLGA-gelatin scaffold. When chondrogenic differentiation of ASCs was evaluated for both constructs, sulfated glycosaminoglycan (sGAG) content was significantly higher in the TGF-β1-immobilized scaffold. This study showed that ASCs containing the TGF-β1-immobilized scaffold better promoted cartilage regeneration in defective articular cartilage, which is assessed by histological observation. Based on the above results, we conclude that TGF-β1-immobilized PLGA-gelatin scaffold seeded with ASCs considerably enhances the quality of the tissue-engineered cartilage, therefore, advancing the field of cartilage tissue engineering.