Open AccessOptimization of Endoglucanase and Xylanase Activities from Fusarium verticillioides for Simultaneous Saccharification and Fermentation of Sugarcane Bagasse
Author(s) -
Maíra Nicolau de Almeida,
Valéria Monteze Guimarães,
Daniel Luciano Falkoski,
Guilherme B. T. Paes,
José Ivo Ribeiro,
Evan Michael Visser,
Rafael Ferreira Alfenas,
Olinto Liparini Pereira,
Sebastião Tavares de Rezende
Publication year - 2013
Publication title -
applied biochemistry and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.558
H-Index - 111
eISSN - 1559-0291
pISSN - 0273-2289
DOI - 10.1007/s12010-013-0572-9
Subject(s) - xylanase , bagasse , cellulase , hydrolysis , fermentation , chemistry , ethanol fuel , food science , biomass (ecology) , cellulose , enzymatic hydrolysis , lignocellulosic biomass , ethanol fermentation , ethanol , central composite design , response surface methodology , microbiology and biotechnology , agronomy , biochemistry , enzyme , chromatography , biology
Enzymatic hydrolysis is an important but expensive step in the production of ethanol from biomass. Thus, the production of efficient enzymatic cocktails is of great interest for this biotechnological application. The production of endoglucanase and xylanase activites from F. verticillioides were optimized in a factorial design (2(5)) followed by a CCDR design. Endoglucanase and xylanase activities increased from 2.8 to 8.0 U/mL and from 13.4 to 114 U/mL, respectively. The optimal pH and temperature were determined for endoglucanase (5.6, 80 °C), cellobiase (5.6, 60 °C), FPase (6.0, 55 °C) and xylanase (7.0, 50 °C). The optimized crude extract was applied in saccharification and fermentation of sugarcane bagasse from which 9.7 g/L of ethanol was produced at an ethanol/biomass yield of 0.19.
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