Open AccessQuantitative Primary Tumor Indocyanine Green Measurements Predict Osteosarcoma Metastatic Lung Burden in a Mouse Model
Author(s) -
Mitchell S. Fourman,
Adel Mahjoub,
Jon B. Mandell,
Shibing Yu,
Jessica C. Tebbets,
Jared A. Crasto,
Peter E. Alexander,
Kurt R. Weiss
Publication year - 2018
Publication title -
clinical orthopaedics and related research
Language(s) - English
Resource type - Journals
eISSN - 1528-1132
pISSN - 0009-921X
DOI - 10.1007/s11999.0000000000000003
Subject(s) - medicine , indocyanine green , osteosarcoma , lung , pathology , radiology , nuclear medicine
Current preclinical osteosarcoma (OS) models largely focus on quantifying primary tumor burden. However, most fatalities from OS are caused by metastatic disease. The quantification of metastatic OS currently relies on CT, which is limited by motion artifact, requires intravenous contrast, and can be technically demanding in the preclinical setting. We describe the ability for indocyanine green (ICG) fluorescence angiography to quantify primary and metastatic OS in a previously validated orthotopic, immunocompetent mouse model.