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Stable isotope characterization of olive oils: II‐deuterium distribution in fatty acids studied by nuclear magnetic resonance (SNIF‐NMR)
Author(s) -
Royer A.,
Naulet N.,
Mabon F.,
Lees M.,
Martin G. J.
Publication year - 1999
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-999-0232-y
Subject(s) - chemistry , deuterium , fractionation , isotope , isotope fractionation , abundance (ecology) , nmr spectra database , nuclear magnetic resonance spectroscopy , mass spectrometry , fatty acid , analytical chemistry (journal) , chromatography , spectral line , organic chemistry , physics , quantum mechanics , astronomy , fishery , biology
Site‐specific isotope fractionation of hydrogen was investigated, at natural abundance, by deuterium nuclear magnetic resonance (SNIF‐NMR) on nearly two hundred olive oil samples. Owing to the complexity of the 2 H‐NMR spectra of the mixtures of fatty acids obtained after hydrolysis of the oils, the different signals were gathered into six clusters. Knowing the contribution to the clusters of each of the four fatty acids considered (C 16:0 , C 18:0 , C 18:1 , and C 18:2 ) and the composition of the fatty acids in the mixture, it is possible to compute the site‐specific isotope ratios of the clusters from the molar fractions obtained from the 2 H‐NMR‐spectra and from the total isotope ratio of the mixture, determined by isotope ratio mass spectrometry (IRMS). The results are discussed in terms of geographical (country, region and elevation) and temporal (year) parameters and they are tentatively explained on a climatic basis.