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Fractionation of soybean phospholipids by high‐performance liquid chromatography with an evaporative light‐scattering detector
Author(s) -
Wang Tong,
Hammond Earl G.,
Cornette James L.,
Fehr Walter R.
Publication year - 1999
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-999-0145-9
Subject(s) - chromatography , high performance liquid chromatography , chemistry , chromatography detector , fractionation , fraction (chemistry) , gas chromatography , phosphatidylethanolamine , fatty acid , soybean oil , phospholipid , phosphatidylcholine , biochemistry , membrane
Abstract Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) from 23 soybean lines with a wide range of fatty acid compositions were resolved into seven fractions by high‐performance liquid chromatography (HPLC). Fraction identities were assigned from fatty acid compositions determined by gas chromatography (GC). A mass detector, i.e., an evaporative light‐scattering detector, was used for HPLC quantification. The detector response was a power function of PC and PE concentrations. Various correction methods were applied to the detector response to obtain the best agreement between phospholipid (PL) fatty acid compositions determined by GC and that calculated from the corrected HPLC fraction percentages. The corrected HPLC fraction composition also was compared with that calculated from stereospecific distribution data using a 1‐random‐2‐random hypothesis. Correlation between PL‐fatty acid and HPLC‐fraction percentages showed that genetic modification of soybean oil composition caused changes in PL species, which alter physical properties and may alter the physiological functions of PL in biomembranes.