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Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry of Vernonia galamensis oil
Author(s) -
Ayorinde Folahan O.,
Elhilo Eisa,
Hlongwane Caswell,
Saeed Kamal A.
Publication year - 1999
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-999-0097-0
Subject(s) - mass spectrometry , analytical chemistry (journal) , chemistry , desorption , chromatography , matrix (chemical analysis) , ionization , organic chemistry , ion , adsorption
Matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight (TOF) mass spectrometry (MS) has been used to identify eight triacylglycerols (TAG) in Vernonia galamensis oil (VO). The TAG include trivernolin, divernoloylstearoylglycerol, divernoloyloleoylglycerol, vernoloyllinoleoylstearoylglycerol, vernoloyllinoleoyloleoylglycerol, and vernoloyldilinoleoylglycerol. Samples for the MALDI/TOF/MS analysis were prepared with alpha‐cyano‐4‐hydroxycinnamic acid (matrix) in an acetonitrile/tetrahydrofuran solvent system. A mol ratio of matrix/VO (44:1) gave reproducible composite spectra, resulting in resolutions greater than 8,000 and signal‐to‐noise ratios of above 1000 for the most abundant molecular species. 1,3‐Dioleoyl‐2‐stearoylglycerol and 1,2‐dioleoyl‐3‐palmitoylglycerol were used as calibration standards.