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Enzymatic modification of triacylglycerols of high eicosapentaenoic and docosahexaenoic acids content to produce structured lipids
Author(s) -
Jennings Brenda H.,
Akoh Casimir C.
Publication year - 1999
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-999-0085-4
Subject(s) - rhizomucor miehei , eicosapentaenoic acid , lipase , chemistry , docosahexaenoic acid , capric acid , fish oil , hexane , solvent , chromatography , substrate (aquarium) , triacylglycerol lipase , fatty acid , enzyme , organic chemistry , polyunsaturated fatty acid , fish <actinopterygii> , biology , lauric acid , ecology , fishery
Immobilized lipase, IM60, from Rhizomucor miehei was used as a biocatalyst for the incorporation of capric acid (C10:0) into fish oil originally containing 40.9 mol% eicosapentaenoic (20:5n‐3) and 33.0 mol% docosahexaenoic (22:6n‐3) acid. Acidolysis was performed with and without organic solvent. Pancreatic lipase‐catalyzed sn ‐2 positional analysis was performed after enzymatic modification. Tocopherol analysis was performed before and after enzymatic modification. Products were analyzed by gas‐liquid chromatography. After a 24‐h incubation in hexane, there was an average of 43.0±1.6 mol% incorporation of C10:0 into fish oil, while 20:5 and 22:6 decreased to 27.8±2.2 and 23.5±1.3 mol%, respectively. The solvent‐free reaction produced an average of 31.8±8.5 mol% C10:0 incorporation, while 20:5 and 22:6 decreased to 33.2±3.3 and 28.3±3.9 mol%, respectively. The effect of incubation time, substrate molar ratio, enzyme load, and added water were also studied. In general, as the enzyme load, molar ratio, and incubation time increased, mol% C10:0 incorporation also increased. The optimal mol% C10:0 incorporation was 41.2% at 48 h for the reaction in hexane and 46.4% at 72 h for the solvent‐free reaction. The highest C10:0 incorporation (65.4 mol%) occurred at a molar ratio of 1:8 (fish oil triacylglycerols/capric acid) in hexane. For the solvent‐free reaction, the optimal mol% C10:0 incorporation (56.1 mol%) occurred at a molar ratio of 1:6. An enzyme load of 10% gave the highest mol% C10:0 incorporation (41.4 mol%) in hexane; the highest incorporation (38.3 mol%) for the solvent‐free reaction occurred at 15% enzyme load. Mol% incorporation of C10:0 declined with increasing amounts of added water. The optimal mol% C10:0 incorporation occurred at 1% added water (47.9 mol%) for the reaction in hexane, and at zero added water for the solvent‐free reaction (21.8 mol%). Fish oil containing capric acid was successfully produced and may be nutritionally more beneficial than unmodified oil.

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