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Practical nuclear magnetic resonance analysis of liquid oil in oilseeds: I factors affecting peak width
Author(s) -
Reaney Martin J. T.,
Tyler Nancy J.,
Brown Keith
Publication year - 1999
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-999-0077-4
Subject(s) - deuterium , spectral line , chemistry , chloroform , analytical chemistry (journal) , solvent , nmr spectra database , spectroscopy , resolution (logic) , acetone , nuclear magnetic resonance , chromatography , organic chemistry , physics , quantum mechanics , astronomy , artificial intelligence , computer science
If proton nuclear magnetic resonance (NMR) spectra of single seeds can be improved, a rapid, low‐cost method of screening seeds for oil composition could be developed for use as a selection tool in plant breeding. NMR spectroscopy was performed on single seeds of borage, flax, and canola to evaluate methods for improving spectra quality (narrowing peak widths and increasing signal‐to‐noise ratio) to a degree necessary to measure differences among seeds in a breeding program. Immersion of seeds in a variety of solvents, including deuterated chloroform, deuterated acetone, deuterated dimethyl sulfoxide (DMSO) and completely fluorinated hydrocarbons (FC‐77), narrowed peaks obtained from seeds when compared with spectra from seeds analyzed in the absence of a solvent. Deuterated chloroform and FC‐77 were free of interfering solvent proton peaks while deuterated acetone and deuterated DMSO contributed interfering peaks. The spectra of dehulled seeds had narrower peak widths than did seeds with hulls. Treatments that decreased seed oil viscosity failed to substantially narrow spectra peak widths of seeds. High magnetic field strength did not improve the spectral quality of seeds, as peak widths increased with field strength. Conversely, low field strength limited resolution of oil spectra. Although the 300 MHz spectrum of vegetable oil had greater resolution (narrower peaks) than the 60 MHz spectrum, spectra of seeds produced at 60 MHz had superior resolution to 300 MHz spectra.