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Separation and characterization of peanut phospholipid molecular species using high‐performance liquid chromatography and fast atom bombardment mass spectrometry
Author(s) -
Singleton J. A.,
Ruan M.,
Sanford J. H.,
Haney C. A.,
Stikeleather L. F.
Publication year - 1999
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-999-0046-y
Subject(s) - chromatography , chemistry , phosphatidylethanolamine , high performance liquid chromatography , phospholipid , phosphatidylcholine , fast atom bombardment , mass spectrometry , elution , fraction (chemistry) , tandem mass spectrometry , chromatography detector , molecular mass , liquid chromatography–mass spectrometry , column chromatography , membrane , biochemistry , enzyme
Total lipid extracts from peanut seed were separated on a silica column into a triacylglycerol fraction and a polar lipid fraction by high‐performance liquid chromatography (HPLC). The polar fraction containing the phospholipids was retained on the precolumn, and the triacylglycerol fraction was eluted to a waste flask by a special valve arrangement. Phospholipids were eluted from the precolumn and separated into various classes on a silica analytical column. Each phospholipid class was manually collected and subsequently subjected to reversed‐phase HPLC in tandem with a fast atom bombardment mass spectrometer. Phosphatidylethanolamine was separated into five molecular species. Phosphatidylinositol and phosphatidylcholine were each separated into six molecular species.