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Borago officinalis oil: Fatty acid fractionation by immobilized Candida rugosa lipase
Author(s) -
SchmittRozieres Murielle,
Vanot Guillaume,
Deyris Valérie,
Comeau LouisClaude
Publication year - 1999
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-999-0004-8
Subject(s) - candida rugosa , chemistry , lipase , amberlite , palmitic acid , linoleic acid , fatty acid , stearic acid , chromatography , oleic acid , polyunsaturated fatty acid , hydrolysis , linolenic acid , fractionation , organic chemistry , biochemistry , adsorption , enzyme
γ‐Linolenic acid ( Z,Z,Z ‐6,9,12‐octadecatrienoic acid), a very important polyunsaturated fatty acid is found in the free fatty acid fraction prepared by the hydrolysis of borage oil. Our aim was to enrich this fraction in γ‐linolenic acid using selective esterification. Candida rugosa lipase was used as catalyst after immobilization on the following ion‐exchange resins: Amberlite IRC50, IRA35, IRA93, and Duolite A7, A368, A568. In every case, immobilization modified the lipae's specificity: palmitic, stearic, oleic, and linoleic acids were preferentially esterified compared to γ‐linolenic acid, thus allowing a γ‐linolenic acid enrichment of 3.0.