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Bioconversion of oleic acid to a series of keto‐hydroxy and dihydroxy acids by Bacillus species NRRL BD‐447: Identification of 7‐hydroxy‐16‐oxo‐9‐ cis ‐octadecenoic acid
Author(s) -
Lanser A. C.
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0335-x
Subject(s) - oleic acid , bioconversion , chemistry , substrate (aquarium) , mass spectrometry , derivative (finance) , chromatography , bacillales , strain (injury) , organic chemistry , stereochemistry , bacteria , biochemistry , biology , bacillus subtilis , ecology , genetics , anatomy , fermentation , financial economics , economics
Fifty‐eight strains of eight Bacillus species were selected from the ARS Culture Collection to investigate their ability to bioconvert oleic acid to unique hydroxy or keto derivative. Addition of 0.5% oleic acid substrate to a whole‐cell culture of Bacillus strain NRRL BD‐447 produced mixtures of mono‐, di‐, and trisubstituted compounds. These products appear to contain combinations of keto and hydroxy groups. One of the major products was isolated by high‐performance liquid chromatography and its structure was determined by gas chromatography‐mass spectrometry and nuclear magnetic resonance to be 7‐hydroxy‐16‐oxo‐9‐ cis ‐octadecenoic acid.