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13 C nuclear magnetic resonance characterization of the reaction products of lamb pregastric lipase‐catalyzed hydrolysis of tributyrylglycerol
Author(s) -
Barton Richard H.,
O’Connor Charmian J.
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0274-6
Subject(s) - lipase , chemistry , hydrolysis , catalysis , nuclear magnetic resonance spectroscopy , reaction rate , enzymatic hydrolysis , nuclear chemistry , enzyme , organic chemistry
Lamb pregastric lipase (LPGL), extracted from the tongue root and epiglottis of suckling lamb, has been used to catalyze the hydrolysis of emulsified tributyrylglycerol. Reactions were generally carried out at 35°C and initial pH 7.0. The speciation of the products of the reaction has been examined by 13 C nuclear magnetic resonance spectroscopy. Varying rates of reaction were produced by increasing the amount of enzyme added relative to the amount of lipid. A system rate parameter, ΔpH/Δ t , which is the change in pH (ΔpH) from time zero to the time when the sample was removed (Δ t ), has been developed. 1,2(2,3)‐Dibutyrylglycerol and 2‐monobutyrylglycerol have been identified as products of LPGL‐catalyzed hydrolysis, while 1,3‐dibutyrylglycerol and 1(3)‐monobutyrylglycerol are products of uncatalyzed acyl transfer reactions.