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Fatty acid bioconversions by Pseudomonas aeruginosa PR3
Author(s) -
Kuo T. M.,
Manthey L. K.,
Hou C. T.
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0240-3
Subject(s) - ricinoleic acid , oleic acid , linoleic acid , chemistry , fatty acid , strain (injury) , chromatography , thin layer chromatography , yield (engineering) , biochemistry , castor oil , biology , materials science , metallurgy , anatomy
The bioconversions of oleic acid, linoleic acid, and ricinoleic acid by Pseudomonas aeruginosa PR3 were investigated. The conversion of oleic acid to 7,10‐dihydroxy‐8( E )‐octadecenoic acid (DOD) was improved to better than 80% yields by modifying the culture medium and reaction parameters. The microbial cultures were stable and retained the same level of DOD production capacity for up to 6 mon as examined herewith. Strain PR3 did not bioconvert linoleic acid. However, strain PR3 converted ricinoleic acid to a novel compound, 7,10,12‐trihydroxy‐8( E )‐octadecenoic acid (TOD), with a yield of about 35%. The product was further metabolized by strain PR3. TOD was purified by thin‐layer chromatography, and its structure was determined by 1 H and 13 C nuclear magnetic resonance and mass spectrometry. This is the first report on the production of 7,10,12‐trihydroxy unsaturated fatty acid by microbial transformation.