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Microbial conversion of palmitoleic acid to 9,12‐hexadecadienoic acid (16:2ω4) by Trichoderma sp. AM076
Author(s) -
Shirasaka Norifumi,
Umehara Takuya,
Murakami Tetsuo,
Yoshizumi Hajime,
Shimizu Sakayu
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0211-8
Subject(s) - palmitoleic acid , mycelium , chemistry , fatty acid , yeast , nuclear chemistry , botany , linoleic acid , organic chemistry , biochemistry , biology
Trichoderma sp. AM076, isolated from a freshwater sample, was found to accumulate 9,12‐ cis ‐hexadecadienoic acid (16:2ω4), when grown with palmitoleic acid (16: 1ω7). Methyl myristate was the best carbon source for the conversion of palmitoleic acid to 16:2ω4. The mycelial 16:2ω4 content reached 17.4 mg/g dry mycelia (443 mg/L) when the fungus was grown in a medium that contained 2.0% methyl myristate, 1.5% yeast extract, and 2.0% methyl palmitoleate, pH 6.0, for 5 d at 28°C with shaking. In both nonpolar and polar lipids from the mycelia, 16:2ω4 was detected as one of the major fatty acids when 16:1ω7 was added. It is probable that 16:1ω7 is converted to 16:2ω4 through the Δ12 desaturation reaction.