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Differential extraction of eleostearic acid‐rich lipid‐protein complexes in tung seeds
Author(s) -
Dyer John M.,
Tang Fuqiang,
Chapital Dorselyn C.,
Lax Alan R.,
Shepherd Hurley S.,
Shih Ding S.,
Pepperman Armand B.
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0113-9
Subject(s) - chromatography , incubation , chloroform , extraction (chemistry) , differential centrifugation , centrifugation , chemistry , fraction (chemistry) , biochemistry , biology
Lipid‐protein complexes were identified in the 104,000× g supernatant fraction of developing tung seeds. Incubation of this fraction with linoleoyl‐CoA promoted an increase of chloroform‐extractable lipids in a time‐dependent manner. High‐performance liquid chromatography analysis indicated that the extracted lipids were similar to mature tung oil triglycerides. Differential extraction using chloroform or chloroform/methanol indicated that linoleoyl‐CoA promoted extraction of pre‐existing lipids rather than de novo synthesis. An increase in extractable lipids was also observed after incubation with proteinase K. Isolation of lipid‐protein complexes by sucrose density centrifugation and analysis of proteins by gel electrophoresis revealed several proteins specifically associated with this lipid fraction.