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Production of 8,11,14,17‐ cis ‐eicosatetraenoic acid (20:4ω‐3) by a Δ5 and Δ12 desaturase‐defective mutant of an arachidonic acid‐producing fungus Mortierella alpina 1S‐4
Author(s) -
Kawashima Hiroshi,
Sakuradani Eiji,
Kamada Nozomu,
Akimoto Kengo,
Konishi Kyoko,
Ogawa Jun,
Shimizu Sakayu
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0084-x
Subject(s) - arachidonic acid , mycelium , polyunsaturated fatty acid , eicosapentaenoic acid , food science , biochemistry , fatty acid , phosphatidylcholine , chemistry , linoleic acid , linseed oil , biology , phospholipid , botany , enzyme , membrane
A Δ5 and Δ12 desaturase‐defective mutant of an arachidonic acid‐producing fungus, Mortierella alpina 1S‐4, produced 8,11,14,17‐ cis ‐eicosatetraenoic acid (20:4ω3) intracellularly when grown with linseed oil. Dihomo‐γ‐linolenic acid was the only C 20 polyunsaturated fatty acid (4.9 wt% of total mycelial fatty acids) other than 20:4ω3. AA and 5,8,11,14,17‐ cis ‐eicosapentaenoic acid were not detected. The mycelial lipids consisted of 82.2% (by mol) triacylglycerol (TG), 7.1% diacylglycerol, 8.9% phospholipids (PL), and 1.9% free fatty acids. The percentage of 20:4ω3 was higher in PL (30.1%) than in TG (11.6%), and highest in phosphatidylcholine (38.9%). Under the optimal conditions with a 5‐L jar fermenter, 20:4ω3 production amounted to 97.4 mg/g dry mycelia with a mycelial yield of 23 g/L on the twelfth day (corresponding to 2.24 g/L medium and 37.1% of total mycelial fatty acids).