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Enzymatic glyceride synthesis in a foam reactor
Author(s) -
Yeh YuChih,
Gulari Erdogan
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0078-8
Subject(s) - lauric acid , glyceride , rhizomucor miehei , glycerol , yield (engineering) , chemistry , lipase , ionic strength , substrate (aquarium) , chromatography , chemical engineering , organic chemistry , fatty acid , triacylglycerol lipase , enzyme , materials science , composite material , aqueous solution , oceanography , engineering , geology
We report the results of our study on Rhizomucor miehei lipase‐catalyzed lauric acid‐glycerol esterification in a foam reactor. A satisfactory yield of glyceride synthesis can be achieved with an unusually high initial water content (50% w/w). We found that product formation could be regulated by controlling foaming. Foaming was a function of the air flow rate, reaction temperature, pH value, ionic strength, and substrate molar ratio. Monolaurin and dilaurin, which constituted nearly 80% of the total yield, were the two dominant products in this reaction; trilaurin was also formed at the initial stages of the reaction. A study of pH and ionic strength effects on an independent basis revealed that they affect the interfacial mechanism in different manners. On varying the ratio of lauric acid and glycerol, only a slight change in the degree of conversion was detected and the consumption rate of fatty acid was approximately the same.