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Specific determination of malonaldehyde by N ‐methyl‐2‐phenylindole or thiobarbituric acid
Author(s) -
Inoue Tadamichi,
Ando Ken,
Kikugawa Kiyomi
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0071-2
Subject(s) - chemistry , hydrochloric acid , thiobarbituric acid , ethylenediaminetetraacetic acid , pigment , nuclear chemistry , chromatography , organic chemistry , lipid peroxidation , antioxidant , chelation
Reactivity of a commercially available test kit (LPO‐586), based on N ‐methyl‐2‐phenylindole, toward aldehydes was characterized and compared with that of thiobarbituric acid (TBA). In hydrochloric acid, LPO‐586 produced a violet pigment with malonaldehyde (MA) but not with other tested aldehydes. In methane sulfonic acid, LPO‐586 produced the violet pigment with MA and 4‐hydroxynonenal (HNE), but not with other tested aldehydes. Pigment formation with MA was not inhibited by other aldehydes, but that with HNE was inhibited by alka‐2,4‐dienals. TBA produced a red pigment with MA but not with other tested aldehydes in hydrochloric acid or in acetate with ethylenediaminetetraacetic acid (EDTA). Both the LPO‐586 test in hydrochloric acid and the TBA test in hydrochloric acid or in acetate with EDTA can be used for specific measurement of MA in oxidized lipid samples.