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Eicosapentaenoic acid and docosahexaenoic acid production potential of microalgae and their heterotrophic growth
Author(s) -
Vazhappilly Rema,
Chen Feng
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0057-0
Subject(s) - phaeodactylum tricornutum , docosahexaenoic acid , eicosapentaenoic acid , heterotroph , food science , algae , biology , mixotroph , botany , chemistry , biomass (ecology) , fatty acid , polyunsaturated fatty acid , biochemistry , bacteria , ecology , genetics
Twenty microalgal strains were investigated in photoautotrophic flask cultures for their potential for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production. The highest EPA proportion (% of total fatty acids) was produced by Monodus subterraneus UTEX 151 (34.2%), followed by Chlorella minutissima UTEX 2341 (31.3%) and Phaeodactylum tricornutum UTEX 642 (21.4%). The highest DHA proportion (% of total fatty acids) was obtained in Crypthecodinium cohnii UTEX L1649 (19.9%), followed by Amphidinium carterae UTEX LB 1002 (17.0%) and Thraustochytrium aureum ATCC 28211 (16.1%). Among the 20 strains screened, the EPA yield was high in M. subterraneus UTEX 151 (96.3 mg/L), P. tricornutum UTEX 642 (43.4 mg/L), Chl. minutissima UTEX 2341 (36.7 mg/L), and Por. cruentum UTEX 161 (17.9 mg/L) owing to their relatively high biomass concentrations. The DHA yield was high in C. cohnii UTEX L1649 (19.5 mg/L) and A. carterae UTEX LB 1002 (8.6 mg/L). Heterotrophic growth of these 20 microalgae was also tested on two different carbon sources, acetate and glucose. All microalgae except Nannochloropsis oculata UTEX LB 2164 showed growth on glucose (5 g/L) under heterotrophic conditions. Twelve of them could grow heterotrophically when acetate (1 g/L) was used as their sole carbon and energy source.