Premium
Efficient lipase‐catalyzed production of tailor‐made emulsifiers using solvent engineering coupled to extractive processing
Author(s) -
Edmundo Castillo,
Valérie Dossat,
Didier Combes,
Alain Marty
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0046-3
Subject(s) - glycerol , lipase , chemistry , adsorption , oleic acid , solvent , substrate (aquarium) , catalysis , butanol , chromatography , silica gel , hexane , organic chemistry , ethanol , enzyme , biochemistry , oceanography , geology
Within the framework of enzymatic biosurfactant synthesis, the development of reaction processes leading to a selective and efficient production is of crucial interest. In this work, we proposed an enzymatic method for producing 1(3)‐monooleoyl‐ rac ‐glycerol using a commercial immobilized lipase, the Lipozyme®. To avoid the blockage of the enzyme by the presence of insoluble glycerol, the latter was adsorbed onto dried silica gel. The selective monoester production (83%) was obtained using 2‐methyl‐2‐butanol amended n ‐hexane (90:10, vol/vol) as reaction medium. The observed decrease in the substrate conversion, in a medium containing 2‐methyl‐2‐butanol, was countered by coupling to the reaction an inline selective recovery method for the produced monoester by adsorption onto a silica gel column located at the outflow of the reaction vessel. This process leads to an efficient monooleoyl glycerol production with a high oleic acid conversion (71%) and to the recovery of a 100% pure monooleoyl glycerol.