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The presence of oxidizing enzyme activities in virgin olive oil
Author(s) -
Georgalaki M. D.,
Sotiroudis T. G.,
Xenakis A.
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0027-6
Subject(s) - polyphenol oxidase , chemistry , lipoxygenase , polyacrylamide gel electrophoresis , sodium dodecyl sulfate , enzyme , gel electrophoresis , chromatography , biochemistry , size exclusion chromatography , protease , molecular mass , peroxidase
Samples of Greek virgin olive oils were examined for the presence of proteins and oxidative enzyme activities. All oil samples tested contained detectable amounts of protein, as well as lipoxygenase and polyphenol oxidase activities. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and size‐exclusion chromatography of olive oil extracts revealed the presence of low‐molecular‐mass (10–40 kDa) silver‐staining and ultraviolet‐absorbing components, respectively. Both lipoxygenase and polyphenol oxidase catalytic activities were heat‐ and protease‐sensitive, and they expressed Michaelis‐Menten kinetics.