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Biotransformation of 12‐hydroxyoctadecanoic acid to 12‐hydroxyoctadecanamide by Bacillus cereus 50
Author(s) -
Huang J K,
Keudell K. C.,
Klopfenstein W. E.,
Wen L.,
Bagby M. O.,
Vesonder R. F.,
Norton R. A.,
Weisleder D.
Publication year - 1997
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-997-0188-8
Subject(s) - bacillus cereus , biotransformation , thin layer chromatography , chromatography , chemistry , mass spectrometry , yeast , gas chromatography , bacillales , gas chromatography–mass spectrometry , cereus , bacteria , organic chemistry , biochemistry , biology , bacillus subtilis , genetics , enzyme
A new microbial isolate ( Bacillus cereus 50) transformed 12‐hydroxyoctadecanoic acid to 12‐hydroxyoctadecanamide when grown aerobically in 1% yeast extract medium at 30°C, shaken at 250 rpm for 2 to 5 d. The compound was purified by thin‐layer chromatography and characterized by infrared, gas chromatography, mass spectrometry, and nuclear magnetic resonance. The yields of 12‐hydroxyoctadecanamide were 9.1 and 21.5% after 2 and 5 d, respectively.