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Synthesis of phosphatidylglycerol from soybean lecithin with immobilized phospholipase D
Author(s) -
Wang XingGuo,
Qiu AiYong,
Tao WenYi,
Shen PeiYing
Publication year - 1997
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-997-0149-2
Subject(s) - phosphatidylglycerol , glutaraldehyde , chromatography , chemistry , lecithin , egg lecithin , phospholipase , nuclear chemistry , phospholipid , biochemistry , enzyme , phosphatidylcholine , membrane
Phosphatidylglycerol (PG) was synthesized from soybean lecithin with the help of phospholipase D (PLD) that was immobilized with calcium alginate gel‐enveloped polyethyleneimine‐glutarald ehyde. The immobilization conditions were optimized with response surface analysis: Ca‐alginate 1.39%, polyethyleneimine 7.78%, glutaraldehyde 1.22%. The conversion of phosphatidylglycerol was 87%. The properties of the immobilized PLD were: optimal temperature 25–30°C, optimal pH 8.2, ratio of ether phase and water phase 1.5–2.5:1 (vol/vol), repeated 15 batches operating with stabilizing conversion percentage of phosphatidylglycerol.