Synthesis of phosphatidylglycerol from soybean lecithin with immobilized phospholipase D | Zendy

Wang XingGuo | Zendy; Qiu AiYong | Zendy; Tao WenYi | Zendy; Shen PeiYing | Zendy

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Synthesis of phosphatidylglycerol from soybean lecithin with immobilized phospholipase D

Author(s) -

Wang XingGuo,

Qiu AiYong,

Tao WenYi,

Shen PeiYing

Publication year - 1997

Publication title -

journal of the american oil chemists' society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.512

H-Index - 117

eISSN - 1558-9331

pISSN - 0003-021X

DOI - 10.1007/s11746-997-0149-2

Subject(s) - phosphatidylglycerol , glutaraldehyde , chromatography , chemistry , lecithin , egg lecithin , phospholipase , nuclear chemistry , phospholipid , biochemistry , enzyme , phosphatidylcholine , membrane

Phosphatidylglycerol (PG) was synthesized from soybean lecithin with the help of phospholipase D (PLD) that was immobilized with calcium alginate gel‐enveloped polyethyleneimine‐glutarald ehyde. The immobilization conditions were optimized with response surface analysis: Ca‐alginate 1.39%, polyethyleneimine 7.78%, glutaraldehyde 1.22%. The conversion of phosphatidylglycerol was 87%. The properties of the immobilized PLD were: optimal temperature 25–30°C, optimal pH 8.2, ratio of ether phase and water phase 1.5–2.5:1 (vol/vol), repeated 15 batches operating with stabilizing conversion percentage of phosphatidylglycerol.

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